The manufacture of this type of product can be either centralized or. It maintains a high drug concentration in the blood or prolongs the duration of drug action 2. A number of pharmaceutical encapsulated products are currently on the market, such as aspirin, theophylline and its derivatives, vitamins, antihypertensive, potassium. Complete biodegradability and the lack of toxic products resulting from the. International journal of chemistry and pharmaceutical sciences 169 and serves only to carry haemoglobin. The purpose of this study was to characterize red blood cell rbc deformability by iron deficiency. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size. Injectable controlled release drug delivery systems asian journal of pharmaceutics octdec 2016 suppl 10 4 s465 few advantages offered by parenteral controlled release systems are as follows. Properties of resealed erythrocyte of novel drug delivery carriers2. Blood contains different cells like erythrocytes rbc, leucocytes wbc and. Various bioactive agents encapsulated in erythrocytes are developed for the slow and sustained release in circulation to allow effective treatment of parasitic diseases. Wornout erythrocytes are removed from cir culation and destroyed in the spleen and liver res, and the break down products are recycled.
Ijipsr international journal of innovative pharmaceutical. Preparation and characterization of hormonesensitive. Erythrocytes based delivery system with their ability to provide controllled and site specific drug delivery will revolutionize disease management. Lyse reseal erythrocytes support efficient parasite growth. Carrier erythrocytes are prepared by collecting blood sample from the organism of interest and separating erythrocytes from plasma. Comparative invitro release of diclofenac sodium gel from different marketed products ks prabha, ch ramakrishna, m srivani, vn priyanka, yb priya international journal of life science and pharma research 2. In fact, in gramnegative bacteria, the activity of. Targeted drug delivery, liposome, nanoparticle, resealed erythrocytes.
Engineered erythrocytes as a novel carrier for the targeted drug delivery. The use of resealed erythrocytes as carriers and bioreactors advances in experimental medicine and biology volume 326. Entrapment by endocytosis endocytosis involves the addition of one volume of washed packed erythrocytes to nine volumes of buffer containing 2. Varun raj vemula 1, swathi thakkalapally 2, chaitanya kumari bairi 3 1 departmentofpharmaceutical chemistry, vikas college of pharmacy, jangaon, warangal. In this communication, we describe the preparation and characterization of hormonesensitive resealed ghosts from turkey erythrocytes. Resealed erythrocytes a specified tool in novel drug delivery system vaishali goel, charanjeet kaur, rainu devi, aman kumar, ujjwal nautiyal himachal institute of pharmacy, paonta sahib, distt. Data are presented which show that these resealed ghosts possess many of the permeability characteris tics of their parent native erythrocytes. Resealed erythrocytes can act as drug carriers and targeting tools as well. The common sequential steps to obtain lysedresealed erythrocytes by. Carrier erythrocytes are preapared by collecting blood sample from the organism of interest and separating erythrocytes from the plasma. Citeseerx document details isaac councill, lee giles, pradeep teregowda.
The enzymes used include pglucosidase, pglucoronidase. The overall process is based on response of these cells underosmotic conditions. Yip r, mohandas n, clark mr, jain s, shohet sb, dallman pr. Resealed erythrocytes technology will remain an active area for the future research. Carrier erythrocytes, resealed erythrocytes loaded by a drug or other therapeutic. International journal of chemistry and pharmaceutical sciences.
The aim of the scaleup process in erythrocytes manufacturing is to encapsulate the therapeutic substance in an efficient and fast way to produce drugloaded erythrocytes with good yield. Upon reinjection, the resealed erythrocytes work as slow circulating depots and thereby targeting the drugs to a reticuloendothelial system res. Reduced drug uptake is the third major mechanism responsible for. Pdf preparation and in vitro evaluation of resealed erythrocytes. Such drugloaded carrier erythrocytes are prepared simply by collecting blood samples from the organism of interest, separating erythrocytes from plasma, entrapping drug in the erythrocytes, and resealing the resultant cellular carriers. However, the problems of exogenous enzyme therapy include a shorter circulation half life of enzymes, allergic reactions, and toxic manifestations. Surfacemodified erythrocytes are used to target organs of mononuclear phagocytic system reticuloendothelial system because the changes in the membrane are recognized by macrophages. Erythrocyte, which is also known as red blood cells, has been extensively studied by scientists for their capability as a potential carrier for the delivery of drugs and drugloaded microspheres. Resealed erythrocytes are prepared by isolating erythrocytes from the plasma of the blood sample of an organism of interest, breakdown of cells using different methods and then entrapping the drug which is to be administered into the body into the erythrocytes by various techniques such as hypotonic haemolysis, hypotonic dilution, hypotonic. However, resealed erythrocytes also can be used to target organs other than those of res. College of pharmacy, teerthanker mahaveer university, moradabad, uttar pradesh, india erythrocytes have been the most interesting carrier and have found to possess great potential in drug targeting. The overall process is based on response of these cells under osmotic conditions 6 advantages of resealed erythrocytes as drug carriers.
Loaded erythrocytes 10% haematocrit, 5 ml are passed through a 23gauge hypodermic needle at a flow rate of 10 mlmin. The pores created by this method are resealed by using 154 mm of nacl. They protect the premature degradation, inactivation and excretion of proteins and enzymes they can target the drugs within reticuloendothelial system they facilitate incorporation of proteins and nucleic acids in eukaryotic cells by cell infusion with rbc. Such drugloaded carrier erythrocytes are prepared simply by collecting blood samples from the organism of interest, separating erythrocytes from plasma, entrapping drug in the erythrocytes, and resealing the resultant. The use of cells as drug delivery system requires that the drug which are normally unable to permeate the membrane, should be made to traverse the membrane without causing any irreversible changes in membrane structure and. Wornout erythrocytes are removed from circulation and destroyed in the spleen and liver res, and the breakdown products are recycled.
Asian journal of pharmaceutical research and development. Erythrocytes as drug carriers in medicine ebook by. The sixth meeting on the use of resealed annealed red blood cells was held in irsee, germany by the international societ. In vitro characterization of resealed erythrocytes drug content packed loaded erythrocytes 0. These ruptured membranes can be resealed by raising the salt concentration to its original isotonic levels and upon incubation, the resealed erythrocytes resume their normal biconcave shape and recover their normal impermeability to both macromolecules and ions. Resealed erythrocytes nowadays, there are 30 and more main drug delivery products in the market. By using various physical and chemical methods cells are broken and drug is entrapped into erythrocytes, finally they are resealed and resultant carriers are then called as resealed erythrocytes. Lysereseal erythrocytes for transfection of plasmodium. By using various methods the cells are broken and the drug is entrapped into the erythrocytes, finally they are resealed and the resultant carriers are then called resealed erythrocytes.
Nowadays, there are 30 and more main drug delivery products in the market. December 1989 the book gives a better understanding of the role of the resealed erthytocytes as potential slow release carriers for drugs and enzymes, being very helpful to scientists working in the fiels as well as to clinicians for treatment improvement. These problems can be successfully overcome by administering the enzymes as resealed erythrocytes. Resealed erythrocytes a specified tool in novel drug. Erythrocytes, also known as red blood cells, have been extensively studied for their potential carrier capabilities for the delivery of drugs and drugloaded microspheres. This study was intended to develop an economical and simple method for transfection of p.
Hence, these carriers are called resealed erythrocytes. Resealed erythrocytes serve as an ideal carrier for antineoplastic agents, antimicrobial drug and vitamins and steroids. The reasons for this increasing interest in drug delivery are due to the increasing need of safe drugs. The parameter indicates the effects of shear force and pressure by which resealed erythrocytes formulations are injected, on the integrity of the loaded cells. Institute of pharmaceutical sciences, udaipur rajasthan 301 erythrocytes, also known as red blood cells, have been extensively studied for their potential carrier capabilities for the delivery of drugs and drugloaded microspheres. Erythrocytes live only about 120 days because of wear and tear on their plasma membranes as they squeeze through the narrow blood capillaries. Applications microparticulate drug delivery offers several applications for drugs having poor bioavailability. Pdf carrier erythrocytes are emerging as one of the most promising biological drug delivery systems investigated in recent decades. Resealed erythrocytes gaining more importance which is due to they have ability to circulate entire body and they are biocompatible, they show zero order release kinetics, complete biodegradability, lack of toxic product, controllable life span. Resealed erythrocytes as carrier for drugs provides secure and more effective drug targeting for lon ger period of time and have advantage over several drugs carrier models regarding the. Contents of the powerpoint on resealed erythrocytes include.
Introduction at present, there are 30 main drug delivery products on the market. Read erythrocytes as drug carriers in medicine by available from rakuten kobo. Pdf resealed erythrocyte based drug delivery system. Resealed erythrocytes, as a drug delivery system has excellent capacity to enhance the therapeutic index and patient compliance. Although drugloaded erythrocytes are biological products, the process can be long and difficult. Resealed erythrocytes are prepared by isolating erythrocytes from the plasma of the blood sample of an organism of interest, breakdown of cells using different methods and then entrapping the drug which is to be administered into the body into the erythrocytes by various techniques such as. Resealed erythrocytes have the following advantages. Introduction basic concept of rbc drug carrying potential of rbc advantages and limitations source and isolation of rbc methods of drug loading in vitro characterization shelf and storage stability. Introduction erythrocytes, the most abundant cells in the human. Nlcs appeared as consumer products first on the cosmetic market. Resealed erythrocytes as a carrier for drug targeting. Resealed erythrocytes as drug carriers pharmatutor.
1480 416 886 792 1058 684 959 1496 466 213 1207 770 1431 1519 13 1485 50 1519 514 873 986 1438 1281 861 999 1143 746 939 109 702 1236 1154 410 869 751 546 1268 173 1436 112 405 1254